The Crabtree Lab
We are interested in the role of epigenetic mechanisms in the development of the nervous system and also in cancer. We focus primarily on the role of an ATP-dependent chromatin remodeling complex called mSWI/SNF or BAF that is frequently mutated in cancer and neurodevelopmental diseases. We are also developing new techniques based on chemically induced proximity to explore and understand how these mechanisms relate to autism, speech disorders and intellectual disability.
Braun SMG, Petrova R, Tang J, Krokhotin A, Miller EL, Tang Y, Panagiotakos G,Crabtree GR. BAF subunit switching regulates chromatin accessibility to controlcell cycle exit in the developing mammalian cortex. Genes Dev. 2021 Mar1;35(5-6):335-353. doi: 10.1101/gad.342345.120. Epub 2021 Feb 18. PMID:33602870; PMCID: PMC7919417.
Lo YH, Kolahi KS, Du Y, Chang CY, Krokhotin A, Nair A, Sobba WD, Karlsson K,Jones SJ, Longacre TA, Mah AT, Tercan B, Sockell A, Xu H, Seoane JA, Chen J,Shmulevich I, Weissman JS, Curtis C, Califano A, Fu H, Crabtree GR, Kuo CJ. ACRISPR/Cas9-Engineered ARID1A-Deficient Human Gastric Cancer OrganoidModel Reveals Essential and Nonessential Modes of Oncogenic Transformation.Cancer Discov. 2021 Jan 15. doi: 10.1158/2159-8290.CD-20-1109. Epub ahead ofprint. PMID: 33451982.
Ni K, Ren J, Xu X, He Y, Finney R, Braun SMG, Hathaway NA, Crabtree GR,Muegge K. LSH mediates gene repression through macroH2A deposition. Nat Commun.2020 Nov 6;11(1):5647. doi: 10.1038/s41467-020-19159-0. PMID: 33159050; PMCID:PMC7648012.