Calvin was a graduate student in the laboratory and defined the transcriptional hierarchy (Nature 355, 457, 1993) the elements of which are mutated in most cases of inherited diabetes. (MODY). He also made the first observation that rapamycin interfered with the pathways that control protein synthesis (Nature 1993), an observation that was later shown to […]
Cor Verwei developed the approach of using small binding sites to regulate indicator genes in transgenic mice (JBC 265, 1990) and showed that NF-AT activity was present in many different cell types. He is presently a Professor at the University of the Netherlands studying autoimmunity. He can be reached at verweij@abcoude.com
Mike discovered that NF-AT was made up of two components, one (NF-ATc) was cyclosporin-sensitive and cytoplasmic and required calcium for activation (Nature 1991). The other component, (NF-ATn) was nuclear and newly induced. Mike also developed the first in vitro transcription system that faithfully reflects T lymphocyte activation requirements (Nature 1991). Mike just took over the […]
Daryl cloned NFATc3 while in the lab and then went to graduate school. He is currently a graduate student in Bioinformatics in the Haussleer lab at ucsc. He can be reached at: daryl@soe.ucsc.edu. You can find out what he is up to at: http://www.soe.ucsc.edu/~daryl/cv/cv.pdf.
One of the first graduate students in the lab. Linda worked with Dirk Mendel to discover and characterize DCoH. She is now with her husband and children living happily at a secure but undisclosed location in Colorado having escaped from all this.
Neil demonstrated that calcineurin was a key component of the lymphyocyte activation pathway and furthermore mapped out the interaction between calcineurin and cyclosporin (Nature 1992). He also developed a way of activating transcription using FK506 to recruit an activation domain to a DNA binding domain (JBC 1993). This was the first use of the two […]
Jamie showed that signals through the IL-2 receptor allowed cells to enter the cell cycle by controlling the degradation of p27. Jamie went to David Morgan’s lab for a post doctoral fellowship. Jamie has the record for the shortest time spent as a graduate student: 3.8 years. She has decided to take some time off […]
David developed the method for regulating protein-protein interactions with synthetic ligands or Chemical Induces of Dimerization (CIDs) (Spencer et al Science 1993). Using this approach he showed that receptors such as the T cell receptor, the B cell receptor, the Fas receptor and many others can be regulated by simple proximity. He worked with Tom […]
Jeff developed the method for purifing NF-AT and later cloned NF-ATc1 and c2 with Steffan Ho. He and Luika Timmerman also showed that NF-ATc family members shuttled into and out of the nucleus and that this shuttling allowed the discrimination of the Ca2+ signals originating from CRAC channels from those originating at IP3 gated intracellular […]
Steffan developed the method of regulating transcription with synthetic ligands (Nature 382, 822, 1996) which was later taken up by Ariad and Jim Wilson’s gene therapy group and used for the first regulated effective gene therapy in primates. In addition, Steffan cloned NF-ATc1, c2 and NF-ATc3 with Jeff Northrop. Finally Steffan found that calicheamicin oligosaccharide […]